Fig. 3: Pp4c is required for development and virulence in Magnaporthe oryzae. | Communications Biology

Fig. 3: Pp4c is required for development and virulence in Magnaporthe oryzae.

From: A multilayered regulatory network mediated by protein phosphatase 4 controls carbon catabolite repression and de-repression in Magnaporthe oryzae

Fig. 3

Colonies (a) and conidiophores (b) of the wild-type, Δpp4c, and PP4C-complemented strain of Δpp4c (pp4c-c). Scale bar = 10 mm (a) and scale bar = 50 μm (b). c Colony diameters of the wild-type, Δpp4c, and pp4c-c. d Conidiation of the wild-type, Δpp4c, and pp4c-c. Strains were cultured on CM at 25 °C for 8 days (c, d). e Conidial germination rates on hydrophobic surfaces under dark conditions at 22 °C for 4 h. f Spores and developing appressoria of the wild-type, Δpp4c, and pp4c-c strains incubated on hydrophobic membranes at 22 °C for 2, 4, 6, 8, and 24 h. Scale bar = 20 μm. g Virulence assays of the wild-type, Δpp4c, and pp4c-c on barley. Scale bar = 10 mm. Virulence assays on rice: representative images (h), and disease index (%) (i). Scale bar = 10 mm. j Invasive growth of the wild-type and Δpp4c on barley leaves. Scale bar = 10 μm. k Penetration rates on barley leaves at 24 and 48 h post-inoculation (hpi). Error bars represent SD. Tukey's HSD test was used for significance testing: *p < 0.05; **p < 0.001. n = 3 biologically independent samples.

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