Fig. 6: Regulation of lipid utilization gene expression by Pp4c, Snf1, and CreA in Magnaporthe oryzae.

Relative expression level of lipase genes (a) and genes involved in fatty acid activation and transport, and β-oxidation (b), ICL1, and MCL1 (c) of ΔcreA in glucose and olive oil media relative to the wild-type. Relative expression level of lipase genes (d) and genes involved in fatty acid activation and transport, and β-oxidation (e), ICL1, and MCL1 (f) of Δsnf1 in glucose and olive oil media relative to the wild-type. Relative expression level of lipase genes (g) and genes involved in fatty acid activation and transport, and β-oxidation (h), ICL1, and MCL1 (i) of Δpp4c in glucose and olive oil media relative to the wild-type. H3 and α-ACTIN were used as reference genes. Error bars represent SD. Asterisks “*” indicate significant differences from the wild-type (Tukey's HSD test, p < 0.05). n = 3 biologically independent samples. j Schematic diagram of lipid metabolism regulation by Smek1, Pp4c, Snf1, CreA, and Crf1 in M. oryzae. Data for Smek1 and Crf1 were obtained from previous studies10,11.