Fig. 4: Effects of drugs on cAMP production in COS-7 cells in the presence and absence of BuChE. | Communications Biology

Fig. 4: Effects of drugs on cAMP production in COS-7 cells in the presence and absence of BuChE.

From: Evidence from a mouse model supports repurposing an anti-asthmatic drug, bambuterol, against Alzheimer’s disease by administration through an intranasal route

Fig. 4

A Effects of bambuterol (1 µM) (solid-green squares) and rivastigmine (1 µM) (solid-black square) on cAMP production in COS-7 cells in the absence of BuChE (basal: white circles). BRET curves and area under the curve (insert) are presented. B, C Effect of tested drugs at 1 µM, alone [rivastigmine (solid-black squares), ICI118551 (solid-black triangles), bambuterol (solid-green squares)] or in combination [rivastigmine + bambuterol (green squares), ICI118551 + bambuterol (green triangles)], on cAMP production in COS-7 cells in the presence of BuChE (5 U/mL). BRET curves (B) and area under the curve (C) [control (white), rivastigmine alone (solid-gray), ICI118551 (hatched gray), bambuterol alone (solid-green), rivastigmine + bambuterol (dark solid-green), ICI118551 + bambuterol (dark hatched green)] are presented. Results are expressed as mean ± SEM (n = 3 (A) and n = 6 (B) technical replicates/condition, a representative experiment is shown). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001 (one-way ANOVA followed by Bonferroni’s test).

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