Fig. 1: Tetracycline-inducible expression in monoclonal cell lines is sufficient for the acquisition of target protein in-cell NMR spectra. | Communications Biology

Fig. 1: Tetracycline-inducible expression in monoclonal cell lines is sufficient for the acquisition of target protein in-cell NMR spectra.

From: Protein structure and interactions elucidated with in-cell NMR for different cell cycle phases and in 3D human tissue models

Fig. 1

A Scheme of the tetracycline-inducible piggyBac construct inserted into HEK293-TRex cells. B Confocal images showing the GFP reporter expression in non-induced HEK293-TRexTR-hSOD1+/Poly cells (control) and HEK293-TRexTR-hSOD1+/Poly and HEK293-TRexTR-hSOD1+/Mono cells induced with tetracycline for 48 h. C Flow cytometry (FCM) plots for non-induced HEK293-TRexTR-hSOD1/Poly cells, and HEK293-TRexTR-hSOD1+/Poly and HEK293-TRexTR-hSOD1+/Mono cells 48 h after expression induction. D, E 1H-15N 2D SOFAST-HMQC in-cell NMR spectra of (pelleted) non-induced HEK293- TRexTR-hSOD1+/Mono cells (background) and HEK293-TRexTR-hSOD1+/Mono cells 48 h after induction, respectively.

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