Fig. 2: The application of stable cell line approach for preparation of in-cell NMR samples of TTHA and CAII. | Communications Biology

Fig. 2: The application of stable cell line approach for preparation of in-cell NMR samples of TTHA and CAII.

From: Protein structure and interactions elucidated with in-cell NMR for different cell cycle phases and in 3D human tissue models

Fig. 2

A1H-15N 2D SOFAST-HMQC in-cell NMR spectra from HEK293-TRexTR-TTHA+/Mono cells, 48 h after expression induction. B The imino regions of 1H 1D in-cell NMR spectra of induced and non-induced (background) HEK293-TRexTR-CAII+/Mono cells. NMR spectra were measured at 37 °C using a bioreactor.

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