Fig. 3: The stably transfected cell lines allow the acquisition of in-cell NMR spectra in distinct cell cycle phases. | Communications Biology

Fig. 3: The stably transfected cell lines allow the acquisition of in-cell NMR spectra in distinct cell cycle phases.

From: Protein structure and interactions elucidated with in-cell NMR for different cell cycle phases and in 3D human tissue models

Fig. 3

A, B Overview of the stable cell line manipulations leading to the acquisition of in-cell NMR spectra (A1) at the G1/S and (B1) G2/M boundary of the cell cycle. The propidium iodide (PI) DNA content staining of G1/S (A2) and G2/M (B2) synchronized HEK293-TRexTR-hCAII+/Mono cells before gel immobilization and NMR analysis, 1H 1D in-cell NMR spectra of G1/S (A3) and G2/M (B3) synchronized HEK293-TRexTR-hCAII+/Mono cells 48 h after expression induction before (black) and after (red) addition of MZA. Signals arising from the zinc-coordinating histidines (H94, H96, and H119) in the active site of CAII are indicated. NMR spectra were measured at 37 °C using a bioreactor.

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