Fig. 4: The stable cell lines allow the acquisition of in-cell NMR spectra from 3D models of human tissue. | Communications Biology

Fig. 4: The stable cell lines allow the acquisition of in-cell NMR spectra from 3D models of human tissue.

From: Protein structure and interactions elucidated with in-cell NMR for different cell cycle phases and in 3D human tissue models

Fig. 4

A1, B1 Overview of the stable cell line manipulations leading to the acquisition of in-cell NMR spectra in 3D cell culture (spheroids); (A2) (Microscope) images of the in-cell NMR sample consisting of HEK293-TRexTR-TTHA+/Mono spheroids (green) embedded in low-melting agarose gel thread, (B2) (Microscope) images of the hybrid in-cell NMR sample consisting of MCF7pKrox24DsRED cells (red; DsRED expression induced by addition of FGF2) and HEK293-TRexTR-CAII+/Mono spheroids (green) embedded in low-melting agarose gel thread; (A3) 1H-15N 2D SOFAST-HMQC in-cell NMR spectrum of spheroid preparation from induced HEK293-TRexTR-TTHA+/Mono cells; (A4) 1H 1D in-cell NMR spectra of HEK293-TRexTR-hCAII+/Mono and (B3) HEK293-TRexTR-hCAII+/Mono::MCF7pKrox24DsRED spheroids before and after treatment with 100 μM CAII inhibitor MZA. NMR spectra were measured at 37 °C using a bioreactor.

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