Fig. 4: CryoDRGN reveals side-to-side assembly of endophilin B1 on lipid bicelles.
From: Peripheral membrane protein endophilin B1 probes, perturbs and permeabilizes lipid bilayers
a 3D reconstruction of particles (758,658 particles; 6.22 Å) used in cryoDRGN heterogeneous ab-initio reconstruction and the resulting UMAP distribution showing locations of different k-means clustered classes and the path of the graph traversal. Symbols mark the different stoichiometries present in each class of particles: (#) four, (†) five, or (‡) six dimers bound per nanodisc. b k-means cluster 0; 72,644 particles; 9.59 Å, c k-means cluster 1; 81,574 particles; 7.42 Å, d k-means cluster 2; 72,337 particles; 8.15 Å, e k-means cluster 3; 80,745 particles; 7.53 Å, f k-means cluster 4; 79,658 particles; 8.03 Å, g k-means cluster 5; 83,754 particles; 7.85 Å, h k-means cluster 6; 73,407 particles; 8.65 Å, i k-means cluster 7; 33,465 particles; 8.54 Å, j k-means cluster 8; 96,256 particles; 6.46 Å, k k-means cluster 9; 84,818 particles; 6.40 Å.
