Table 4 Permeabilization assay using endophilin B1 and liposomes of different lipid compositions (lipid ratio shown as molar percentage) where n is the number of replicates
From: Peripheral membrane protein endophilin B1 probes, perturbs and permeabilizes lipid bilayers
Name | Lipid composition | Protein conc. (μM) | Temperature (°C) | n | Calcein release (%) |
|---|---|---|---|---|---|
Mix 1 | 100% DOPC | 0.5 | 25 | 18 | 0.68 ± 0.14 |
Mix 2 | 55% DOPS, 40% DOPE, 5% Cholesterol | 0.5 | 25 | 18 | 6.9 ± 0.3 |
Mix 3 | 50% DOPS, 40% DOPE, 5% Cholesterol, 5% 18:1 cardiolipin | 0.5 | 25 | 18 | 10 ± 1 |
Mix 4 | 55% DOPS, 40% DOPE, 5% 18:1 cardiolipin | 0.5 | 25 | 18 | 11 ± 1 |
Mix 5 | 40% DOPS, 40% DOPE, 20% 18:1 cardiolipin | 0.5 0.5 5 5 | 25 37 25 37 | 9 9 6 6 | 11 ± 1 27 ± 2 65 ± 6 96 ± 3 |
