Fig. 4: Effect of different carbon sources on BDQ-induced growth inhibition rescue by ASCT expression.

The effects of ASCT on transgenic M. smegmatis treated with various concentrations of BDQ were investigated by culturing cells in minimal media (HdeB) supplemented with various carbon sources: 22.5 mM malate, 30 mM glycerol, 45 mM acetate, 22.5 mM succinate, or 15 mM glucose. Asterisks indicate statistically significant differences (p ≤ 0.05, Welch’s t-test) in ASCT strains compared to the control (pYUB28b). Results represent means ± SD from three biological replicates per experiment.