Fig. 2: Structural similarities of the top- and bottom-strand nicking domains of BsmI (left) with FokI and EcoRI catalytic domains (right). | Communications Biology

Fig. 2: Structural similarities of the top- and bottom-strand nicking domains of BsmI (left) with FokI and EcoRI catalytic domains (right).

From: Crystal structures of monomeric BsmI restriction endonuclease reveal coordinated sequential cleavage of two DNA strands

Fig. 2

The catalytic residues are shown in stick representation. The sequence motifs of the catalytic site are shown at the bottom. For clarity, the structural alignments were only performed between the residues [1-25, 74-142, 164-185] of BsmI (bottom strand nicking domain) and the residues [35-114, 141-170, 201-210, 235-239] of EcoRI on the one hand, and between the residues [469-499, 520-571, 597-633] of BsmI (top strand nicking domain) and the residues [417-496, 514-547] of FokI on the other hand. The RMSD was obtained with the MatchMaker tool of ChimeraX49.

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