Fig. 1: Generation of MOR FRET-based conformation sensor. | Communications Biology

Fig. 1: Generation of MOR FRET-based conformation sensor.

From: Dynamics of agonist-evoked opioid receptor activation revealed by FRET- and BRET-based opioid receptor conformation sensors

Fig. 1

A Schematic overview of the rat MOR. Different sites were chosen to insert a sYFP into the intracellular loop 3, after M264 (blue), L265 (green), S266 (red) or G267 (magenta). The C-terminus was shortened after T370 (violet) or T364 (gray, short), and an mTurq2 was appended. Scheme created with BioRender (Agreement Number: JP270G30ZL). B Scatter dot-plot showing the relative change of the FRET emission ratio induced by agonist application on the different MOR sensor constructs. C Representative picture of HEK293 cells stably expressing the MOR FRET sensor G267 in the YFP channel. D Averaged FRET-based single-cell recording of the MOR conformation sensor with sYFP inserted after G267 and mTurq2 inserted after T370. Application of DAMGO induced a robust decrease in FRET emission ratio by about 6% (mean ± SEM; n = 8).

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