Fig. 2: Phenotype of ventricular fibroblasts from 4-week-old control and Scn5a+/ΔQKP mice. | Communications Biology

Fig. 2: Phenotype of ventricular fibroblasts from 4-week-old control and Scn5a+/ΔQKP mice.

From: Long QT syndrome type 3 gain-of-function of Nav1.5 increases ventricular fibroblasts proliferation and pro-fibrotic factors

Fig. 2

a Representative xCELLigence cell index time course (fibroblasts seeded at 5000 cells/well, 5 days after cell isolation) and proliferation rate between 20 and 25 h post-seeding for fibroblasts from heart of control and Scn5a+/ΔQKP mice (n = 52 & 38, respectively), normalized to control mean value. b Representative immunostainings of Ki67 (red) in left ventricular sections of control and Scn5a+/ΔQKP mice (scale bar: 50 µm), and the ratio of Ki67-positive nuclei (n = 20 & 11, respectively). c Representative western blot and protein expression in fibroblasts from control and Scn5a+/ΔQKP hearts of cleaved caspase 3 / caspase 3 (n = 14 & 9, respectively), and Mcl-1 (n = 9 & 6, respectively). d Representative immunostainings of α-smooth muscle actin (α-SMA, green) in fibroblasts from control and Scn5a+/ΔQKP hearts, 15 hours post-seeding (scale bar: 100 µm). e Acta2 (gene encoding α-SMA) mRNA expression relative to Hprt and normalized to control mean value (2-ΔΔCt; n = 10 & 8, respectively). f Representative western blot and protein expression, in cardiac fibroblasts from control and Scn5a+/ΔQKP mice, of α-SMA (n = 16 & 11, respectively) and periostin (POSTN; n = 21 & 15, respectively). Data obtained from 4-week-old mice. The median and interquartile range are shown. The dots indicate the values for each independent mouse. *, **, ***p < 0.05, 0.01 and 0.001, respectively (Mann-Whitney test).

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