Fig. 1: Functional validation of oxidative decarboxylases from WRF in the bacterium P. putida KT2440. | Communications Biology

Fig. 1: Functional validation of oxidative decarboxylases from WRF in the bacterium P. putida KT2440.

From: Metabolic profiling of two white-rot fungi during 4-hydroxybenzoate conversion reveals biotechnologically relevant biosynthetic pathways

Fig. 1

The figure shows 4-hydroxybenzoic acid (4-HBA) conversion to hydroquinone (HQ) by WRF and to protocatechuic acid by P. putida KT2440, as well as metabolite concentrations in the supernatant of cultivations conducted with engineered P. putida expressing oxidative decarboxylases from G. subvermispora (GS) and T. versicolor (TV). Bars represent the average concentration from biological triplicates and error bars show the standard deviation. Dot-plots depict individual data points. Media = abiotic media; KT2440= wild-type P. putida; \(\Delta\)pobAR + EV = P. putida KT2440 with pobAR knockout and empty vector.

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