Fig. 4: Intracellular metabolomic profiles for various 4-HBA conversion pathways in T. versicolor and G. subvermispora.

Heatmaps below each metabolite display log₂fold changes of relative metabolite abundance in four pairwise comparisons (from left to right): AO vs. without AO (NAO) in agitation (Ag), AO vs NAO in static (St) conditions, St vs. Ag with AO, and St vs. Ag without AO. G. subvermispora (GS) is shown in the top row and T. versicolor (TV) in the bottom row. Green and dark blue colors are indicative of a negative and positive log2fold change, respectively, in each pairwise comparison. Asterisks (*) within heatmap boxes show significant differences in pairwise comparisons, confirmed by a Tukey’s honest significant difference test. A metabolite is considered as present if detected in at least three biological replicates. Gray boxes indicate the metabolite is not detected. Molecules without heatmaps do not have commercially available standards. Continuous and discontinuous black lines correspond to validated and proposed enzymatic steps, respectively. Metabolomics results originate from three biological replicates for each cultivation condition. AO ascorbic acid and α-tocopherol antioxidants, NAO no antioxidants, Ag agitation, St static, GS G. subvermispora, TV T. versicolor.