Fig. 5: Proposed phenylpropanoid biosynthetic pathway in T. versicolor.

a Metabolomic analysis in the extracellular milieu of T. versicolor for a group of mass features including cinnamic acid, p-coumaric acid, caffeic acid, ferulic acid, phenylalanine, and tyrosine. The heatmap, represented by the log10 of peak heights, shows relative metabolite abundances from cultivations conducted in different conditions. A metabolite is considered present if it is detected in at least three biological replicates. Data originated from three biological replicates. b Intracellular proteomic analysis showing log10 concentrations of putative enzymes of the proposed phenylpropanoid biosynthetic pathway. Reference enzymes for each reaction (from 1 to 10) are color-coded to highlight their origins:blue for plants, red for bacteria, and yellow for yeast. This heatmap represents the same conditions as in section A. Data originated from three biological replicates. c Proposed phenylpropanoid biosynthetic pathway in T. versicolor. The quantification of identified metabolites in the extracellular fraction is shown in bar graphs and the results derive from averages of biological duplicates. Error bars indicate the absolute difference between the biological duplicates. Dot-plots depict individual data points. The enzymes of each reaction are: Reaction 1:PAL L-phenylalanine ammonia-lyase, PTAL bifunctional L-phenylalanine/L-tyrosine ammonia-lyase, reaction 2:C4H cinnamate 4-hydroxylase, 3:C3H 4-coumarate 3-hydroxylase, reaction 4:Fcs feruloyl-CoA synthase, reaction 5:Ech enoyl-CoA hydratase/lyase, reaction 6:Vdh vanillin dehydrogenase, reaction 7:3-oxoacyl-(acyl-carrier protein) reductase, reaction 8: 3-oxoacyl CoA thiolase, reaction 9:Alpha/beta hydrolase family, reaction 10: COMT caffeate/5-hydroxyferulate 3-O-methyltransferase. Ag agitation, AO antioxidants, NAO no antioxidants, St static;controls:AOt0 = Time zero control (with seed media from T. versicolor), AOtn = 7-day control (with seed media from T. versicolor), NAOt0 = Time zero control (without seed media), NAOtn = 5-day control (without seed media), (no seed) NAOTn = 7-day control (without seed media and without antioxidants). Supplementary Data 6 includes source data underlying charts of Fig. 5c.