Fig. 1: Developmental progression in cloned wildtype (WT) porcine embryos.

a The morphological overview of WT porcine embryos at E41, E62, and E90, demonstrating a developmental progression in physical form and size. Scale bar, 5 cm. b–d Parasagital scans of magnetic resonance imaging (MRI) at E41 (b, b′, b″), E62 (c, c′, c″), and E90 (d, d′, d″) for detailed visualization of organ development and evaluation of fetal morphometric parameters. Scale bar, 1 cm (b, b′, b″), 2 cm (c, c′,c″), and 4 cm (d, d′, d″). e–g Representative H&E staining of hindlimb sections across gestational stages E41 (e), E62 (f), and E90 (g). High magnification images of the boxed area are presented in (e′, f′, g′). Scale bar, 1 mm (e, f, g) and 250 µm (e′, f′, g′). h–j Representative Masson’s Trichrome staining demonstrates the progression of connective tissue development within the muscle across the same developmental stages E41 (h), E62 (i), and E90 (j). Boxed areas are enlarged in (h′–j′). Scale bar, 1 mm (h, i, j) and 250 µm (h′, i′, j′).