Fig. 5: Differential expression analysis between E41 MYF5/MYOD/MYF6-null and E41 WT porcine hindlimbs.
From: Porcine myogenesis in cloned wildtype and MYF5/MYOD/MYF6-null porcine embryo
a Volcano plot of differentially expressed genes (DEGs). Red dots indicate upregulated genes in MYF5/MYOD/MYF6-null hindlimbs and blue dots indicate downregulated genes in MYF5/MYOD/MYF6-null hindlimbs. The cut-off criteria for assessing the DEGs was |log2FC|>1 and adjusted p < 0.05. b, c Gene ontology (GO) pathway analysis highlights pathways, signaling, and development-related terms of upregulated genes in MYF5/MYOD/MYF6-null hindlimbs (b) and downregulated genes in MYF5/MYOD/MYF6-null hindlimb samples (c). The x-axis represents the counts of genes in each GO term and the color scale shows the increased significance of biological processes using the over-representation test with an adjusted p < 0.05. d The heatmap represents upregulated and downregulated genes in the WT hindlimbs vs. the MYF5/MYOD/MYF6-null hindlimbs at E41. The heatmap color scheme key is provided, with red representing upregulated and blue representing downregulated genes.
