Fig. 1: Concentration methods have limited impact on stool EV derived protein and RNA recovery. | Communications Biology

Fig. 1: Concentration methods have limited impact on stool EV derived protein and RNA recovery.

From: The proteomic landscape of stool-derived extracellular vesicles in patients with pre-cancerous lesions and colorectal cancer

Fig. 1

A Representative transmission electron microscopy images showing EVs that were isolated by SEC and further concentrated by ultrafiltration (10 kDa and 100 kDa) at 80kx (scale bar: 200 nm), with white arrows indicating individual EVs. B Total particle recovery as measured by MRPS for the different concentration methods, Mean ± SD, Friedman Test. C Bar chart showing the percent of particles that fall within a given particle size range. D Percent vesicular RNA among concentration methods, Mean ± SD, Friedman Test. E Total protein yield (μg) across the different EV concentration methods measured by BCA assay, Mean ± SD, Friedman Test, **p < 0.01. F Ratio of total particle number and total protein yield, a higher ratio suggests less co-isolated soluble protein contamination, Mean ± SD, One Way ANOVA, *p < 0.05.

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