Fig. 3: Co-isolation of zymogen granules containing pancreatic enzymes in stool EV preparations. | Communications Biology

Fig. 3: Co-isolation of zymogen granules containing pancreatic enzymes in stool EV preparations.

From: The proteomic landscape of stool-derived extracellular vesicles in patients with pre-cancerous lesions and colorectal cancer

Fig. 3

A Number of proteins with hydrolase activity detected among the different concentration methods and larger cohort, Mean ± SD. B Detection range for hydrolases in the larger cohort of 63 stool EV samples. C Percent of total protein abundance associated with GI secreted hydrolases among the different concentration methods and larger cohort, Mean ± SD. D Pie chart indicating tissue specificity/enrichment of hydrolases detected in larger cohort. E Dot blot showing stool-derived EV sample blocked either with or without Tween-20 and incubated with pancreas associated primary antibodies. Additionally western blot was performed using the same antibodies with pancreatic juice derived EVs being used as a positive control. F GP2 concentration in stool and pancreatic juice measured by flow cytometry, Mann–Whitney Test, ****p < 0.0001, the boxes and the whiskers in this plot and hereafter indicate the first and third quartiles, the medians, and the minimum and maximum values, respectively. G Percent GP2 positive EVs in stool and pancreatic juice measured by flow cytometry, Mann–Whitney Test, **p < 0.01.

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