Fig. 4: Colon-enriched/specific proteins identified in stool EV preparations.

A Percent of total protein abundance associated with colon enriched/specific proteins among the different concentration methods and larger cohort, Mean ± SD. B Western blot (15ug) showing colon enriched protein expression among the five concentration methods and pre-isolation sample (stool supernatant). The positive control is from a sample that was submitted for mass spectrometry. C Subcellular location for colon enriched/specific proteins identified in the larger cohort of 63 stool EV samples. D String diagram depicting interactions of proteins specific/enriched in the colon. E Detection range for colon enriched/specific proteins in the larger cohort of 63 stool EV samples. F CEACAM5 concentration in stool measured by flow cytometry. G Percent CEACAM5 positive EVs in stool measured by flow cytometry.