Fig. 5: Bacterial proteins identified in stool-derived extracellular vesicles.

A Most abundant species’ in EV preparations from patient 3, UF 10 kDa sample. B Most abundant species in EV preparations from patient 4, UF 10 kDa sample. C Western blot (15ug) showing bacterial EV protein expression post-isolation for the five concentration methods and pre-isolation sample (stool supernatant). D Total human and bacterial protein abundances for each patient and concentration method. E Total human and bacterial protein abundances for each patient and concentration method after the top four human secreted proteins were removed from the calculation.