Fig. 5: Relative stability of Enctb subunits along the edges of the encapsulin intermediates.
From: In situ and in vitro cryo-EM reveal structures of mycobacterial encapsulin assembly intermediates
Enctb subunits are coloured according to their cross-correlation with their density map. a Models and EM density maps of the 48-, 52- and 54-mers of the encapsulin shell shown with the open side facing forward. Scalebar: 25 Å. Examples of less well (b) and well resolved Enctb monomers (c) overlaid with the density map. Scalebars: 10 Å. (d) Model of the 54-mer of the encapsuling shell shown from the side. Lower cross-correlation values are observed near the edge of the open side (Yellow and orange) whereas higher cross-correlation values are observed on the far-side (pink and purple). Scalebar: 25 Å. e Models of the 48- and 52-mers of the encapsulin shell. These intermediates primarily differ by the addition of two monomer pairs, which increases the cross-correlation values of monomers that were previously on the edge of the partial encapsulin (black arrows). Monomers shown in panel (d) and (e) are marked with grey dashed squares. Scalebar: 25 Å.
