Table 1 Cryo-EM Single Particle Analysis Data collection and modelling information
From: In situ and in vitro cryo-EM reveal structures of mycobacterial encapsulin assembly intermediates
60-mer | 48-mer | 52-mer | 54-mer | |
|---|---|---|---|---|
EM data collection/processing | ||||
EMDB ID | EMD-13164 | EMD-51500 | EMD-52340 | EMD-52341 |
Microscope | FEI Titan Krios | |||
Acceleration voltage (KV) | 300 | |||
Camera (mode) | GATAN K3 (counting) | |||
Number of fractions | 40 | |||
Exposure time (s) | 1.8 | |||
Total exposure (e-/Å2) | 40 | |||
Defocus range (µm) | 0.7–2 | |||
Pixel size (Å) | 0.834 | |||
Magnification | 163000 | |||
Number of micrographs | 3827 | |||
Initial reference model | 6I9G (PDB) | in silico | in silico | in silico |
Number of particles (final map) | 34,427 | 3427 | 4378 | 6294 |
Number of symmetry operators | 60 | 37 | 37 | 26 |
Global resolution (Å) | 2.29 | 5.42 | 4.51 | 4.58 |
Modelling | ||||
PDB ID | 7P1T | 9GOT | 9HQ7 | 9HQC |
Bond lengths RMSZ | 0.37 | 0.37 | 0.37 | 0.37 |
Bond Angles RMSZ | 0.57 | 0.57 | 0.57 | 0.57 |
Clashscore | 2 | 7 | 6 | 5 |
Ramachandron Favoured | 98% | 98% | 98% | 98% |
Ramachandron Allowed | 2% | 2% | 2% | 2% |
Ramachandron Outliers | 0 | 0 | 0 | 0 |
Sidechain outliers | 1% | 1% | 1% | 1% |
