Fig. 2: Variations in cell and nucleus morphologies arise from uneven cell division.

A Images of live cells illustrating cell division and growth. A mother cell 1 h before division (left). Fuchsia outline denotes mother cell contour. Daughter cells possess different sizes immediately upon division (middle). Yellow outline denotes the larger daughter cell while orange outline denotes the smaller daughter cell. Daughter cells 6 hr after division (right). Scale bar = 15 μm. B Cell area evolution of mother and daughter cells shown in (A) illustrates that the area difference increased during cell growth. C Corresponding nucleus size evolution shows that the nucleus area differences remained during cell growth. D Cell areas at 0 and 6 h after division are correlated. N  = 116. p < 0.0001. 95% confidence interval is [0.431, 0.679]. E Nucleus areas at 0 and 6 h after division are correlated. N = 116. p <  0.0001. 95% confidence interval is [0.452, 0.694]. F Histogram of cell areas 0 and 6 h after division. Inset shows that the normalized cell area PDFs of the 0 h and 6 h data can be described by the same log-normal distribution (black dashed line). G Corresponding histogram of nucleus areas 0 and 6 h after division. Inset shows that the normalized nucleus area PDFs of the 0 h and 6 h data can be described by the same log-normal distribution (black dashed line). H Nucleus-cell area correlation immediately upon division. N = 116. p <  0.0001. 95% confidence interval is [0.628, 0.803]. I Nucleus-cell area correlation 6 h after division. Black lines in (D, E, H, I) represent best linear fits. N = 116. p <  0.0001. 95% confidence interval is [0.738, 0.865]. 3 biological replicates were used for all analyses.