Table 2 Intra-assay variation of each sequencing method

From: Double and single stranded detection of 5-methylcytosine and 5-hydroxymethylcytosine with nanopore sequencing

 

Intra-assay RMSD at x depth (%)

Mean count of sites compared across replicates

5x

10x

15x

5x

10x

15x

Nanopore

5mC

17.8

16.8

15.3

24,840,932

19,873,534

9,765,218

5hmC

13.3

12.5

11.5

24,840,932

19,873,534

9,765,218

oxBS-seq

20.0

14.1

8.4

1,838,247

22,292

3437

TAB-seq

16.3

11.3

6.3

2,813,280

105,536

6126

  1. Intra-assay variation shown for Nanopore sequencing (N = 4), oxBS-seq (N = 2), and TAB-seq (N = 3). Root Mean Square Deviation (RMSD) is calculated pairwise between all possible replicate pairs. The mean of these pairwise comparisons is shown here for different depths.