Fig. 5: Results of ELISA to examine the specificity of the obtained antigen-specific clones.

a Screening ELISA after magnetic beads selection using rIgG conjugated magnetic beads. The results for representative clones from GdAID² (left) and mAID² (right) derived libraries are shown. RIgG and ovalbumin (OA) were used as a positive and negative control antigen, respectively. b ELISA to examine the specificities of anti-rIgG clones. Results for representative clones obtained from GdAID² derived library (left) and mAID² (right) are shown. Apoferritin (Apo), EGFR-Fc and OA were used as negative control antigens. c ELISA to screen the anti-Apo clones. The results for representative clones from GdAID² (left) and mAID² (right) derived libraries are shown. Apo and OA were used as a positive and negative control antigen, respectively. d Screening ELISA after 3 rounds of panning from mAID² derived libraries using Apo conjugated magnetic beads, followed by limiting dilution cloning. Data for representative clones are shown. Apo and OA were used as a positive and negative control antigen, respectively. e ELISA to examine the specificity of anti-Apo clone. Results for the representative clone obtained from GdAID² (left) and mAID² (right) derived library is shown. The mAID² derived clone (Anti-Apo#1(m)) is the one isolated after panning. Note that the results of the anti-Apo#4(Gd) and anti-Apo#1(m) were obtained from separate, independent experiments. RIgG, EGFR-Fc and OA were used as negative control antigens. f ELISA to screen the anti-EGFR clones. The results for representative clones from GdAID² (left) and mAID² (right) derived libraries are shown. EGFR-Fc and recombinant human IgG (rhIgG) were used as a positive and negative control antigen, respectively. g ELISA to examine the specificities of anti-EGFR clones. Results for representative clones obtained from GdAID² (left) and mAID² (right) derived libraries are shown. RIgG, Apo, OA and rhIgG were used as negative control antigens.