Fig. 6: Sequence analysis of the V regions of antigen-specific clones.
From: Monoclonal antibody generation by controlled immunoglobulin gene rearrangements
a VL sequences of Anti-rIgG#2(Gd) (derived from GdAID2) and anti-rIgG#1(m) (derived from mAID2) (ELISA results are shown in Fig. 5b) are compared with the parental VL sequence (VL of parental GdAID2 and mAID2 cells). Dots indicate the identical nucleotides to those of parental VL whereas dashes represent deletions. Pink and blue boxes represent CDRs and FRs, respectively. Note that the red “G” in the CDR1 of parental VL represents the insertion that confer frame-shift. The horizontal bars represent gene conversion tracts and corresponding pseudogenes are described besides (pV11 etc.). b VL sequences of Anti-Apo#4(Gd) and Anti-Apo#1(m) (ELISA result is shown in Fig. 5e) are compared with the parental VL sequence. c VL sequences of Anti-EGFR#1(Gd) and anti-EGFR#2(m) (ELISA results are shown in Fig. 5g) are compared with the parental VL sequence. d VH sequences of Anti-rIgG#2(Gd) and anti-rIgG#2(m) are compared with the parental VH sequence. e VH sequence of Anti-Apo#4(Gd) and Apo#1(m) are compared with the parental VH sequence. f VH sequences of Anti-EGFR#1(Gd) and anti-EGFR#2(m) are compared with the parental VH sequence. All the sequences have been registered in GenBank, with the accession numbers PQ885560 to PQ885571.
