Fig. 7: OVCH1-AS1 locus and conservation.
From: The transcriptomic landscape of monosomy X (45,X) during early human fetal and placental development
a Overview of chromosome 12 (p.11.22) and genes located in the region of OVCH1-AS1. Arrows indicate direction of transcription. The red arrow indicates OVCH1-AS1 (non-coding RNA gene) and blue arrows indicate protein coding genes. b Multiz alignments of species across the OVCH1-AS1 locus. A single line indicates no transcript in the aligned species. Double lines show aligning species having one or more unalignable transcripts in the gap region. c Relative expression of all chromosome 12 genes in 45,X tissues compared to 46,XX (orange) and 46,XY (green). The higher expression of OVCH1-AS1 in monosomy X tissue is indicated as log2 fold change (FC) in relation to control tissues. d Violin plot of OVCH1 in the different tissues (normalized counts from bulk RNA-sequencing) (left panel) (n = 4 samples in each group). Quantitative real-time PCR (polymerase chain reaction) showing relative expression of OVCH1 in fetal kidney (right panel) (n = 4 samples each group). Data are presented as scatter dot plots with mean and standard error of the mean also shown. e Violin plot of ERGIC2 in different tissues (bulk RNA-seq) (left panel) (n = 4 samples in each group). Quantitative real-time PCR showing relative expression of ERGIC2 in fetal pancreas (right panel) (n = 4 samples each group). ns, not significant.
