Fig. 1: Identification of phage genes involved in Tmn evasion.
From: Phage engineering to overcome bacterial Tmn immunity in Dhillonvirus
a Genomic comparison of ΦSMS22 and ΦSMA8. b Bar graph summarizing the results of spot assays assessing the infectivity of phages ΦSMS22 and ΦSMA8 on bacterial strains equipped with the Tmn defense system. The y-axis represents the logarithm of the efficiency of plating (EOP), calculated as the ratio of PFU/mL for bacteria carrying the Tmn defense system to the PFU/mL for bacteria carrying empty vectors. The x-axis indicates the names of the phages. The experiment was performed in triplicate, and the bar graph shows the mean values with error bars representing standard deviations. c Bar graph based on the measurement of phage infectivity against Tmn defense system by deletion of ORF35 (anti-Tmn) in ΦSMS22 phage. The deletion mutant phages were synthesized using the in vitro synthesis method, and three independent phages (ΦSMS22Δanti-Tmn_1, ΦSMS22Δanti-Tmn_2, ΦSMS22Δanti-Tmn_3) were obtained after reboot and used for spot assay. d Phylogenetic tree of anti-Tmn homologs from diverse bacterial genera (GA: 20, coverage > 40%, identity ≤95%). e Genomic comparison of ΦSMS22, ΦSMA8, ΦSMW22, ΦSMA2, ΦKSS9, ΦSMS24, and ΦDruSM5. f Bar graph summarizing the results of a phage spot assay assessing the infectivity of the seven phages against bacteria with Tmn defense system. g Bar graph based on spot assay of phage on bacteria with Tmn and anti-Tmn or anti-Tmn homologs.
