Fig. 3: The expression of FABP5 increases in mesothelioma cell lines. | Communications Biology

Fig. 3: The expression of FABP5 increases in mesothelioma cell lines.

From: FABP5 is a key player in metabolic modulation and NF-κB dependent inflammation driving pleural mesothelioma

Fig. 3

A Total RNA of IST-Mes-2, MPP89, and HMC35 cells was analyzed by quantitative real-time PCR for the expression of the key fatty acid transporters Slc27a1, Slc27a4, Slc27a5, Fabp1, Fabp3, Fabp4, Fabp5, and CD36. Values were normalized using GAPDH as the housekeeping gene. Data were statistically analyzed by Student’s t-test and are reported as mean values ± SE of three independent experiments. B Total RNA of IST-Mes-2, MPP89, and HMC35 cells stimulated or not with TNF-α for 24 h was analyzed by quantitative real-time PCR for the expression of FABP5. Values were normalized using GAPDH as the housekeeping gene. Data were statistically analyzed by Student’s t-test and are reported as mean values ± SE of three independent experiments. The asterisk indicates statistically significant differences between mesothelioma cell lines and primary mesothelial cells. C Volcano plot indicates the gene expression profile of MPP89 mesothelioma compared to normal mesothelial HMC35 cells. Lower expressed genes were indicated with blue, while higher expressed genes were indicated with red.

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