Fig. 1: NLRC5 is upregulated in MI cardiomyocytes and its cardiomyocyte knockdown leads to enlarged infarct size and worsened cardiac dysfunction. | Communications Biology

Fig. 1: NLRC5 is upregulated in MI cardiomyocytes and its cardiomyocyte knockdown leads to enlarged infarct size and worsened cardiac dysfunction.

From: Targeting NLRC5 in cardiomyocytes protects postinfarction cardiac injury by enhancing autophagy flux through the CAVIN1/CAV1 axis

Fig. 1

a Boxplot showing NLRC5 expression in cardiomyocytes at different sites of the infarcted heart in the human multi-omics database. IZ indicates Ischemic Zone, BZ indicates Border Zone, and RZ indicates Remote Zone. b Western blot and quantification of NLRC5 expression levels in border zone heart tissue lysates from mice at different time points post-MI (n = 5 each). c Western blot and quantification of NLRC5 expression in AC16 human cardiomyocytes undergoing oxygen-glucose deprivation (OGD) treatment at different time points (n = 4 each). d Western blot and quantification of NLRC5 expression in neonatal mouse cardiomyocytes (NMCMs) undergoing OGD treatment at different time points (n = 3 each). e Quantitative RT-PCR analysis and quantification of Nlrc5 mRNA levels in NMCMs undergoing OGD treatment at different time points, with Actb as a control (n = 4-5 each). f The experimental timeline for the MI model. Vehicle (Ad5-Ctrli) or Ad5-cTnT-NLRC5i were injected to peri-infarct myocardium of adult mice during MI surgery. Echocardiographic analyses were performed at day (D) 1, D7, D14, and D28 post-MI (dpi). Hearts were harvested at 7, 14, and 28 dpi for histological analyses. g Western blot and quantification of NLRC5 in border zone heart tissue lysates from MI mice treated with Ad5-Ctrli or Ad5-cTnT-NLRC5i at 7, 14, and 28 dpi (n = 4 each). h Representative echocardiographic images of MI mice treated with Ad5-Ctrli or Ad5-cTnT-NLRC5i at 1 and 28 dpi. i Quantitative analysis of LVEF, LVFS, LVID;d, and LVID;s in MI mice treated with Ad5-Ctrli or Ad5-cTnT-NLRC5i at 1, 7, 14, and 28 dpi (n = 8 per group). j Infarct area in Ad5-Ctrli and Ad5-cTnT-NLRC5i treated hearts presented by triphenyltetrazolium chloride (TTC) staining of heart tissues at 7 dpi and corresponding quantification (n = 6 each). Scale bar, 2 mm. k Regional scar area in Ad5-Ctrli and Ad5-cTnT-NLRC5i treated hearts shown by Masson trichrome staining of heart tissue sections at 28 dpi and corresponding quantification (n = 6 each). Scale bar, 1 mm. Data are presented as mean ± SD. a Group-wise Mann-Whitney test. b-e 1-way ANOVA with Tukey multiple comparisons test. g, j, k Standard unpaired Student t test. i two-way repeated-measure ANOVA with Sidak post-hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001. ns indicates not significant.

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