Fig. 1: Effects of VA on cancer cachexia parameters in CT26-induced cachectic mice. | Communications Biology

Fig. 1: Effects of VA on cancer cachexia parameters in CT26-induced cachectic mice.

From: Regulating Sirtuin 3-mediated mitochondrial dynamics through vanillic acid improves muscle atrophy in cancer-induced cachexia

Fig. 1

A Experimental scheme of the in vivo study is shown. The animals were subcutaneously inoculated with 5 × 105 CT26 colorectal carcinoma cells in the dorsal region, except for the NC group. VA administration (100 mg/kg of body weight) via oral gavage started one week after cancer injection. The control groups (NC group and CT26 group) were administered distilled water. The vehicle or VA was fed five times per week for three weeks. The artwork was created on biorender.com. B, C Changes in body weight and final body weight were measured. D After sacrificing the mice, tumor-free body weight was calculated by subtracting the tumor weight from the final body weight. E Average food and water intake were measured. F Tissue weights of the heart, lung, BAT, liver, and spleen were measured. G Serum levels of CRP, IL-6, and TNF-α were measured. All data are expressed as mean ± S.E.M. (n = 6). Statistical differences were calculated by one-way ANOVA with post hoc Tukey’s test. #p < 0.05 vs. NC mice; *p < 0.05 vs. CT26 mice. VA vanillic acid, NC normal control, BAT brown adipose tissue.

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