Fig. 3: Effects of VA on MAFbx, MURF1, and AKT expression in TA of CT26-induced cachectic mice. | Communications Biology

Fig. 3: Effects of VA on MAFbx, MURF1, and AKT expression in TA of CT26-induced cachectic mice.

From: Regulating Sirtuin 3-mediated mitochondrial dynamics through vanillic acid improves muscle atrophy in cancer-induced cachexia

Fig. 3

TA was immunostained with antibodies for A MAFbx and B MURF1. The slides were counter-stained with DAPI for visualization of the cell nucleus (magnification ×400, scale bar = 75 μm). C Average fluorescence intensity was quantified using ImageJ software and presented as a graph. D, E Protein levels of D proteolytic markers (MAFbx, MURF1, AKT) and E inflammation markers (STAT3, NF-κB) were measured in TA by western blot analysis. Results were expressed relative to GAPDH. All values are the means ± S.E.M. of three or more independent experiments. Statistical differences were evaluated using an unpaired t-test and a subsequent post hoc one-tailed Mann–Whitney U test. #p < 0.05 vs. NC mice; *p < 0.05 vs. CT26 mice. VA vanillic acid, NC normal control, TA tibialis anterior.

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