Fig. 2: SiMPull-POP captures FKBP dimerization.
From: Cholesterol inhibits assembly and oncogenic activation of the EphA2 receptor
a Schematic representation of DIBMALPs containing Myr-FKBP-GFP (monomer, left). FKBP-GFP dimerization is induced by the AP ligand (dimer, right). b Experimental step distribution of FKBP-GFP photobleaching in control conditions (black) and in the presence of AP ligand (red). c Calculated oligomeric distribution corrected for 70% maturation efficiency of GFP. p-values are from two-way ANOVA followed by Tukey multiple comparison test, ***, p ≤ 0.001; ****, p ≤ 0.0001. n = 3 biologically independent experiments. Bar graphs represent mean ±S.D.
