Fig. 3: Cholesterol reduction promotes EphA2 oligomerization in the absence of ligand.
From: Cholesterol inhibits assembly and oncogenic activation of the EphA2 receptor
a Schematic of DIBMALP containing an EphA2-GFP monomer. b Step distribution of control DIBMALPs (black) or those formed from cells treated with EA1 (pink), MβCD (blue) or both (magenta). c Oligomeric distribution calculated from data in panel b. d Schematic representing DDM micelles containing EphA2-GFP. e Step distribution of DDM-solubilized EphA2-GFP in the same conditions as in DIBMALPs. f Oligomeric distribution of DDM-solubilized EphA2-GFP photobleaching data. p-values are from two-way ANOVA followed by Tukey multiple comparison test.*, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001. n≥3 biologically independent experiments. Bar graphs represent mean ±S.D.
