Fig. 1: Combined use of split scaffold RNA and ssDNA activators enabling RNA detection by Cas12a. | Communications Biology

Fig. 1: Combined use of split scaffold RNA and ssDNA activators enabling RNA detection by Cas12a.

From: Precise amplification-free detection of highly structured RNA with an enhanced SCas12a assay

Fig. 1

a Schematic illustration of the SCas12a assay by using a 20-nt scaffold RNA and ssDNA activator for RNA detection. b Schematic representation of Cas12a activated by combinations of scaffold RNA, ssDNA activator, and RNA target. c Extension of the scaffold RNA, ranging from 23 to 32 nt (S3 to S12), to detect RNA at PAM-distal region.

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