Fig. 3: Detection of distinct regions within an HCV fragment by Cas12a complexed with S6 scaffold and ssDNA activator.
From: Precise amplification-free detection of highly structured RNA with an enhanced SCas12a assay
a Schematic of an HCV RNA fragment with four target regions. The predicted secondary structure was determined by NUPACK software. b Heat map representing fold change with respect to NTC at t = 60 min of an in vitro trans-cleavage fluorescence assay using the scaffold S3 to S8. c Comparison of the different scaffold RNAs (S3 to S12) and ssDNA activators for HCV target. The plot represents the fold change in fluorescence intensity normalized to the NTC at t = 60 min (n = 3). Supplementary Data 1 are provided as a Supplementary Data 1 file.
