Fig. 9: Utilization of the SCas12aV2 assay for pathogen detection.

a Schematic diagram of differentiating ATCC25922 E. coli viable and dead bacteria by SCas12aV2 assay. b The ratio of viable bacteria to total bacteria was verified using standard plate counts as 0, 1, 5, 10, 50, and 100%, respectively. c Analyzing viable pathogenic bacteria using the SCas12aV2 assay. The fluorescence response of SCas12aV2 was assessed for profiling bacteria at various percentages of viable bacteria (0, 1, 5, 10, 50, and 100%). The reaction employed E. coli 16S rRNA primer1, 1 μM E. coli 16S rRNA primer2, and E. coli 16S rRNA targeted S6.1 activator, respectively. d Schematic diagram of detecting SARS-Cov-2 from the clinical swab samples by SCas12aV2 assay. e Pre-extracted RNA from five nasopharyngeal swabs confirmed positive for SARS-CoV-2 by RT-qPCR was tested by SCas12aV2 assay. A confirmed negative swab was tested for comparison. All fluorescence values were normalized to those of WT activator. Statistical analysis for n = 3 biologically independent replicates comparing the normalized fold change for DETECTR vs SCas12aV2 assay. Statistical analysis was conducted using a two-tailed t-test. Statistical significance was determined as follows: ns (not significant) for p > 0.05, * for p ≤ 0.05, ** for p ≤ 0.01, *** for p ≤ 0.001, and **** for p ≤ 0.0001. error bars represent mean value ± SD (n = 3). Supplementary Data 1 are provided as a Supplementary Data 1 file.