Fig. 5: Regulation of viral replication by the dsRNA interactome. | Communications Biology

Fig. 5: Regulation of viral replication by the dsRNA interactome.

From: Cellular dsRNA interactome captured by K1 antibody reveals the regulatory map of exogenous RNA sensing

Fig. 5

a Schematics HCoV-OC43 infection in A549-Cas9 cells with a specific dsRBP candidate expression depleted. b, c Comparison of IFNB1 mRNA (b) and secreted IFN-β (c) levels between poly(I:C) stimulation and HCoV-OC43 infection 48 h.p.i. d, e Comparison of selected ISG mRNA induction between poly(I:C) stimulation (d) and HCoV-OC43 infection 48 h.p.i (e). For (b)–(e), statistical significances were calculated using one-sided Student’s t-test; **p < 0.01 and ***p < 0.001. f Change in nsp12 gRNA and N sgRNA levels measured by RT-qPCR in the indicated KO cells 48 h.p.i. In all bar graphs, an average of three biological replicates are shown with error bars denoting s.e.m. For (f), viral RNA levels from sgNT transduced cells served as the control. Statistical significances were calculated using a one-way ANOVA with Dunnett’s post-hoc test; **adjusted p < 0.01 and ***adjusted p < 0.001.

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