Fig. 6: Arf6 regulates endocytic recycling of PMCA4b.
From: The calcium pump PMCA4b promotes epithelial cell polarization and lumen formation
a, b Transfection of GFP-PMCA4b-expressing MCF-7 and HEK-293 cell lines with wild-type (WT) and Q67L mutant form of Arf6. White arrows point to colocalizing areas. c CD147 immunostaining of non-treated and 24 or 48 h NAV2729-treated GFP-PMCA4b-expressing MCF-7 cells. Insets show magnified areas indicated by the dashed white rectangles. d Statistical analysis of the distribution of cells with internalized GFP-PMCA4b in control and after 48 h of NAV2729 treatment. Data were collected from 8 fields of view from 3 independent experiments; n(PMCA4b control) = 589, n(PMCA4b + 48h NAV2729) = 435 cells. Data were analyzed with chi-square test, p value: ***p < 0.001; non-significant difference is not labeled. e Statistical analysis of the GFP-PMCA4b signal distribution between cytoplasm and plasma membrane. Graph displays means with 95% confidence intervals; data were collected from 3 independent experiments, n(PMCA4b control) = 40, n(PMCA4b + 48h NAV2729) = 40 cells, and analyzed as described in the Methods section. Data were analyzed with unpaired t test, p value: ***p < 0.001. f A scheme depicting the role of Arf6 in PMCA4b trafficking. RE recycling endosome.
