Fig. 9: Drosophila melanogaster (dm)PMCA regulates lumen size and morphology in larval salivary gland.

a DmPMCA immunostaining of the salivary gland of a 3rd stage larva. On the left: upper cell layer section; on the right: middle section. b Co-immunostaining of D. melanogaster Dlg and (dm)PMCA of the salivary gland of a 3rd stage larva. c Western blot analysis of dmPMCA expression in control and ubiquitous daughterless promoter-driven dmPMCA-specific RNAi-expressing 3rd stage D. melanogaster larvae. d Phalloidin staining of control and salivary gland-specific forkhead promoter-driven dmPMCA RNAi-expressing 3rd stage larvae. Images in the lower row show distal parts of the lumen in the same salivary gland as above using higher magnification. e Toluidine blue-stained semi-thin cross-sections of salivary glands from 3rd stage control and forkhead promoter-driven dmPMCA specific RNAi-expressing larvae. The lumen is outlined by red line. f Statistical comparisons of the lumen area ratios on semi-thin cross-sections. Graph displays means with 95% confidence intervals, n = 10 animals. Data were analyzed with Mann–Whitney test; p values: ^nsp ≥ 0.05, **p < 0.01. g Transmission electron microscope images from cross-sections of salivary gland of control and salivary gland specific forkhead promoter-driven dmPMCA RNAi-expressing 3rd stage larvae. Magnified areas indicated by the dashed black rectangles are shown on the right. The lumen is outlined by red line. L lumen.