Fig. 5: Assessing the functional states of tumor infiltrating-B cells and TAMs in PPLELC.

a Subclustering of tumor-infiltrating B cells on the UMAP plots of the snRNA-seq datasets. b Expression and distribution of canonical B cell marker genes among cells. Red to gray: high to low expression. c Dot plot showing the highest expressed genes of each tumor-infiltrating B cell cluster in the snRNA-seq. Dot size indicates the fraction of expressing cells, colored based on normalized expression or activity. d Subclustering of TAMs on the UMAP plots of the scRNA-seq datasets. e Expression and distribution of canonical TAM marker genes among cells. Red to gray: high to low expression. f Dot plot showing the highest expressed genes of each TAM cluster in the snRNA-seq. Dot size indicates the fraction of expressing cells, colored based on normalized expression or activity. g Heatmap showing the expression of MHC molecules, chemokines, cytokines, and other related genes in each TAM cluster. h Boxplots showing the M1 and M2 signature scores for each TAM cluster in the snRNA-seq data. The signature score was calculated by the VISION method. i, j Boxplot showing the angiogenesis and phagocytosis signature scores for each TAM cluster in the snRNA-seq data. The signature score was calculated by the VISION method. k Dot plot showing the expression of the immune costimulatory, checkpoint, and evasion genes for each TAM cluster in the snRNA-seq dataset.