Fig. 6: Ligand-receptor-based interaction between tumor and immune cells.

a Circo plots of the numbers of interaction between cell types in the snRNA-seq data. b, c Chord plots displaying the pathways enriched in the putative ligand-receptor interactions among malignant cell clusters and other cell types. d Dot plots showing the mean interaction strength for selected ligand–receptor pairs between tumor cells and tumor-infiltrating T clusters. Dot size indicates the p value, colored by the average expression level of interaction. e Dot plots showing the mean interaction strength for selected ligand–receptor pairs between tumor cells and TAM clusters. Dot size indicates the p value, colored by the average expression level of interaction. f Dot plots showing the mean interaction strength for selected ligand–receptor pairs between tumor cells and tumor-infiltrating B clusters. Dot size indicates the p value, colored by the average expression level of interaction. g Representative images of mIHC in PPLELC to prove the interaction between tumor cells and T cells. CD8, CD44, LAMA3 and DAPI were labeled with different colors. h Representative images of mIHC in PPLELC to prove the interaction between tumor cells and macrophages. CD68, COL4A5, CD44 and DAPI were labelled with different colors. i Representative images of mIHC in PPLELC to prove the interaction between tumor cells and B cells. CD19, CD74, APP and DAPI were labelled with different colors.