Fig. 4: Genetic Validation of the Brunello CRISPR Knockout Screen.
From: Comprehensive genomic dependency landscape of a human colon cancer organoid
a, b Library-1 guides depletion effect in F147T and F130T compared to the plasmid. X-axis shows guide frequencies in pDNA. Y-axis shows guide frequencies in tumors. Red color represents gRNAs targeting DepMap common essential genes included in the mini library. Blue color represents gRNAs targeting all the other genes in the mini library. c, d Comparison of library-1 guides depletion effect in TP53-KO vs TP53-WT in F147T (MSI) and F130T (MSS). X-axis shows guide frequencies in TP53-WT tumors. Y-axis shows guide frequencies in TP53-KO tumors. Red points are significantly depleted guides, defined as those that were more than one standard deviation away from the diagonal line of identity. e, f The data from same experiment are plotted in two different ways. Guides depletion effect in both tumors compared to the pDNA (e), and comparison between two tumors (f).
