Fig. 3: SSADH deletion only mildly affects parasite development. | Communications Biology

Fig. 3: SSADH deletion only mildly affects parasite development.

From: Metabolic crosstalk between the mitochondrion and the nucleus is essential for Toxoplasma gondii infection

Fig. 3

a SSADH was localized in the mitochondria of T. gondii, co-localized with the mitochondrial marker protein HSP60. b A 7-day plaque assay using the DiCre and Ī”ssadh strain (100 tachyzoites/well, 3 wells/strain). c The relative size of plaques from panel (b). d The replication of Ī”ssadh. The number of parasites in the vacuole was recorded after 24ā€‰h of culture in HFF cells (meanā€‰Ā±ā€‰SEM; two-way ANOVA). e Virulence test of ICR mice infected with DiCre and Ī”ssadh parasites (100 tachyzoites/mouse and 8 mice/strain). f Replication efficiency of the Ī”scsĪ± and Ī”scsĪ±Ī”ssadh strains. The number of vacuoles was counted 24ā€‰h post-infection. g A 10-day plaque assay of the Ī”scsĪ± and Ī”scsĪ±Ī”ssadh strains (100 tachyzoites/well, 3 wells/strain).

Back to article page