Fig. 6: Toxoplasma expresses MDH-FAD in the mitochondrion. | Communications Biology

Fig. 6: Toxoplasma expresses MDH-FAD in the mitochondrion.

From: Metabolic crosstalk between the mitochondrion and the nucleus is essential for Toxoplasma gondii infection

Fig. 6

a Immunofluorescence assays were performed on the 10ƗHA-tagged strain to determine the localization of MDH-FAD. HSP60 was used as a marker specific to the mitochondrion for colocalization. b Toxoplasma MDH-FAD cDNA was amplified and cloned into the pRS416. The parental (BY4741) and ScĪ”mdh1 strains transformed with the empty plasmid (pRS416) or construct (pRS416-MDH-FAD) were characterized on medium with glucose or glycerin as a sole carbon source by serial dilution assay. The plates were grown at 30ā€‰Ā°C for 3 days. c The TgMDH-FAD expressing mini gene was reintroduced into the chromosome of the ScĪ”mdh1. Growth of parental (BY4741), ScĪ”mdh1 and ScĪ”mdh1-TgMDH-FAD strains was tested either on glucose (YPD, fermentable carbon source) or on glycerol (YPG, non-fermentable carbon source) for 3 days at 30ā€‰Ā°C.

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