Fig. 9: Functional analysis of the ME domain.

a The ME comprises the N-terminal, Tudor1, Tudor2, and C-terminal domains. The nuclear localization signal (NLS) of ME was analyzed using https://www.novoprolabs.com/tools/nls-signal-prediction. b The domains were expressed in the UPRT locus of miniAID-ME to create the comp-ME, comp-ME-N/C, and comp-ME-N mutants. c The expression of ME-Ty, ME-N/C-Ty and ME-N-Ty were confirmed by IFA using an anti-Ty antibody. d Plaque assays were carried out under −/+500 μM IAA for 7 days. e The parasites were subjected to intracellular replication assays under specified conditions. (n = 3 independent assays, means ± SEM; ****p ≤ 0.0001, two-way ANOVA).