Fig. 4: WGMS performed on post-lung function tested 13-week-old female lung homogenates from each group (n = 7–8 per treatment group). | Communications Biology

Fig. 4: WGMS performed on post-lung function tested 13-week-old female lung homogenates from each group (n = 7–8 per treatment group).

From: Developmental air pollution exposure augments airway hyperreactivity, alters transcriptome, and DNA methylation in female adult progeny

Fig. 4

OVA treatment significantly induces differentially methylated regions (DMRs; assessed by EdgeR w/ threshold: p < 0.000001, ∆DNAm > |10%|) in Sham (A) and PM2.5 (B) offspring. Significant DMRs denoted in colour (red = decrease in DNAm; blue = increase in DNAm). C Set operations analysis showed how many of those DMRs were shared (AAD DMRs; dark purple), unique to Sham (Sham; red), and unique to PM2.5 (PM2.5; light blue) offspring. The chi-squared analysis found that maternal insult significantly altered DEG expression patterns (p < 0.0001, df = 2). D–G Bubble plots demonstrating DMRs enriched in key Reactome pathways (p < 0.05) DMRs up (D) and downregulated (E) in AAD and those up (F) and downregulated (G) in Sham.

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