Fig. 2: Maternal high-fat diet disrupts intestinal barrier and upregulates LRRC19 expression in offspring.

A Goblet cells in the colon were assessed by PAS staining between MCD, MHFD groups. Scale bar = 50 µm. B The production of MUC2 in the colon was assessed by immunostaining. Scale bar = 50 µm. In (A, B), the number of positively stained cells in each crypt/villus of five random fields from ×200 images was counted. n = 5 mice per group. C The relative expression of Muc2 in mRNA levels was assessed by Realtime-PCR. n = 5 per group. D The relative expression of LRRC19 was assessed in mRNA levels and by immunostaining. n = 4–5 mice per group. Scale bar = 100 µm. MCD, maternal control diet. MHFD, maternal high-fat diet. All data are displayed as means ± SDs. *p < 0.05, **p< 0.01, ***p < 0.001, ****p < 0.0001.